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EGF-loaded Chitosan to Facilitate Healing and Prevent Scar Formation of Cesarean Wound

EGF-loaded Chitosan to Facilitate Therapeutic and Forestall Scar Formation of Cesarean Wound

Cesarean part (CS) is a significant surgical intervention that impacts girls at childbearing age. Scarring from CS is predicted however definitely not fascinating, doubtlessly causes discomfort and psychological misery. Rising proof signifies that epidermal progress issue (EGF) performs essential roles in wound therapeutic with the potential of minimizing scar formation. This examine goals to research the impact of microencapsulated recombinant human EGF (Me-EGF) in scar prevention. Silicone gel was included as a part of the routine scar remedy.

Supplies:

The investigational dressing on this examine: the microencapsulated polysaccharide and oligopeptide-1 is supplied by Good-Care Biotech, Ltd. (model NewEpi®: 10 mcg Me-EGF/ml, Taipei, Taiwan). The investigational dressing NewEpi® was formulated in spray answer with a proprietary drug supply system wherein rhEGF was encapsulated in chitosan as nanoparticles.

Silicone-based scar remedy has proven the useful results of enhancing the looks of scars. Dermatix® (Dermatix® Extremely, Menarini Asia-Pacific, Taiwan) is a Taiwan-FDA accepted topical silicone gel and commercially accessible. It’s clear, odorless and fast drying. Regarding the applicability over decrease stomach space, silicone gel (Dermatix®) was chosen to be a part of the routine care on this examine.

 

Wholesome girls scheduled for cesarean supply had been enrolled and randomized to 3 teams: (1) no scar remedy, (2) silicone gel solely, or (3) silicone gel plus Me-EGF. Vancouver Scar Scale (VSS: vascularity, pigmentation, elasticity, and peak) was used for scar evaluation on the sixth month and ninth month after CS.

Eligibility was decided on the screening go to. Particulars of the examine relating to eligibility, function, examine process, scope of care and potential dangers had been defined. Upon enrollment, full medical historical past of the contributors was obtained, together with historical past of hypertrophic scar or keloid.

Scars from earlier accidents or surgical incisions had been evaluated and recorded. Every affected person was randomized to one of many three remedy arms: Group 1 (receiving no scar remedy), Group 2 (silicone gel solely), and Group 3 (silicone gel plus Me-EGF).

 

Enrolled contributors underwent Cesarean supply carried out by one of many 4 obstetricians on the Division of Obstetrics and Gynecology of CGMH, as a part of their prenatal care. Aside from routine surgical wound care, contributors in Group 1 didn’t obtain any remedy pertaining to scar prevention.

The day of Cesarean supply was recorded as Day 0. For contributors in Group 3, on day Zero previous to remaining dermal closure, 4ml of Me-EGF (containing 40mcg microencapsulated polysaccharide and rhEGF) was sprayed evenly alongside the incision web site, subsequently coated with antibiotic ointment and sterile gauze.

One other 0.5 ml (5 mcg of Me-EGF) was sprayed throughout dressing change on day 1 and day 5 respectively. At every dressing change, the sutured wound was cleaned by sterile regular saline, adopted by Me-EGF sprays, and waited for 2 minutes to permit for absorption, then coated with dry sterile gauze.

Every participant in Group 2 and Group3 introduced residence two tubes of silicone gel once they returned for his or her follow-up visits on day 14. They had been instructed to start out making use of silicone gel evenly to the wound twice a day for 2 months.

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ICP Instrument Check Standard 10

CALMIX10-500 500ML
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ICP Instrument Check Standard 3

CALMIX3-100 125ML
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ICP Instrument Check Standard 4

CALMIX4-100 125ML
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CALMIX4-500 500ML
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CALMIX7-100 125ML
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LCS-4826 1ML
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1000 ug/mL Maltodextrin in HPLC Grade Water Volume: 1mL Ampule**ANALYZED BY LC INSTRUMENTATION**

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ICP-MS Instrument Calibration Standard 1

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1000 ug/mL Isoxaben in HPLC Acetonitrile Volume: 1mL Ampule**ANALYZED BY LCMS INSTRUMENTATION**

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1000 ug/mL Pymetrozin in HPLC Acetonitrile Volume: 1mL Ampule**ANALYZED BY LCMS INSTRUMENTATION**

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1000 ug/mL Clethodium in HPLC Acetonitrile Volume: 1mL Ampule**ANALYZED BY LCMS INSTRUMENTATION**

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CL-ICS-3 125ML
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CL-ICS-4 125ML
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CL-ICS-5 125ML
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1000 ug/mL Norflurazon in HPLC Acetonitrile Volume: 1mL Ampule**ANALYZED BY LCMS INSTRUMENTATION**

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1000 ug/mL Dimethomorph in HPLC Acetonitrile Volume: 1mL Ampule**ANALYZED BY LCMS INSTRUMENTATION**

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Automatic Nucleic Acid Extraction Instrument(32)

VNP-32P 1
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Automatic Nucleic Acid Extraction Instrument(96)

VNP-96 1
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MiniVac Plastic Tubing Instrument to Bottle Lid

ARG1242 EACH
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LCS-1150-ACN 1ML
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ICMIX2-100 125ML
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Blood PCR Kit

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20-abx098092
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Blood bag plug

D1 PK1000
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Defibrinated Horse Blood-400mL

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MED1302 EACH
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M1143-200 each
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Research Members:

This examine is accepted by the InstitutionalReviewBoard at Chang-Gung Memorial Hospital (CGMH, Linkou, Taiwan). All practitioners and analysis staffs are in compliance with Good Scientific Observe tips. A complete of 60 females scheduled for cesarean delivery might be recruited by their main obstetricians on the Division of Obstetrics and Gynecology of CGMH.

 

Previous to their enrollment, the examine procedures might be defined to acquire written knowledgeable consents. The inclusion standards are:

(1) Age of 18 or older;

(2) deliberate for cesarean supply with Pfannenstiel-incision, which is one frequent methodology of performing Caesarian sections in the present day;

(3) had not obtained any remedy for scars at the very least one month earlier than enrollment; and

(4) able to understanding examine protocol with the intention to signal the knowledgeable consent voluntarily.

Sufferers might be excluded if:

(1) presently taking any treatment that may have an effect on end result analysis of this examine, together with however not restricted to: systemic corticosteroids or immunosuppressants;

(2) presently present process any remedies involving decrease stomach, or planning to have such remedies;

(3) having energetic an infection involving stomach on the time of recruitment;

(4) concomitant extreme or poorly-controlled sickness (e.g., cardiovascular, renal, hepatic, pulmonary or gastrointestinal illness, malignancy or historical past of HIV an infection);

(5) with any belly tumor;

(6) present process pressing cesarean supply with out correct pre-op commonplace process, or

(7) having recognized allergy to any of the substances in any of the merchandise used on this examine.

Scientific Analysis and End result Measures:

There have been a complete of 4 time factors at which information had been collected and pictures taken utilizing the identical digital digital camera with mounted settings: day 0 (completion of suture on Cesarean incision), day 1, day 5 earlier than discharge from the hospital, and at follow-up visits in month 3, month 6, and month 9.

After enrollment and earlier than day 0, baseline pictures of previous Cesarean scars had been taken from contributors who had prior Cesarean childbirth, along with pictures of different scars from those that had reported historical past of hypertrophic scar or keloid. Scars had been assessed utilizing Vancouver Scar Scale.

The VSS consists of Four parameters: vascularity (0-3), pigmentation (0-3), elasticity (0-5), and peak (0-3). Previous to the examine graduation, all personnel concerned had obtained necessary coaching on scar evaluation and VSS scoring.

At month 3, 6, and 9, a nurse practitioner took pictures and evaluated the incision scar on every affected person with VSS. Photographs of every therapeutic wound had been taken with a ruler positioned adjoining to the scar to measure the peak raised above pores and skin stage.

Subsequently all scores had been validated twice by completely different analysis staffs by utilizing the pictures on report. No important discrepancies had been discovered. At every follow-up go to, the first obstetrician additionally assessed every affected person for any aspect impact or opposed occasion.

Statistical Evaluation:

Clinicaldata and all scar measurements had been recorded on case report varieties and entered in Microsoft Excel database, later validated by completely different analysis staffs as the ultimate scores. Knowledge had been analyzed utilizing jamovi statistical software program (Model 0.9).

Means and commonplace deviations had been offered for interval variables of baseline traits. Categorical information at baseline had been offered in frequency and analyzed with Chi-Sq. checks. Nonparametric Kruskal-Wallis checks had been carried out on VSS scores for between group comparisons.

A p-value < 0.05 was thought-about statistically important

Keratocan (KERA) Antibody

abx026920-400ul 400 ul
EUR 627.6

Keratocan (KERA) Antibody

abx026920-80l 80 µl
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Keratocan Antibody / KERA

F54366-0.08ML 0.08 ml
EUR 140.25
Description: The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).

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F54366-0.4ML 0.4 ml
EUR 322.15
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CLIA kit for Human KERA (Keratocan)

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  • 10 plates of 96 wells
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Keratocan (KERA) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

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Description: tissue homogenates, cell lysates and other biological fluids.

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EUR 646.7
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EUR 475.34
Description: tissue homogenates, cell lysates and other biological fluids.

Human Keratocan (KERA) ELISA Kit

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EUR 679.04
Description: tissue homogenates, cell lysates and other biological fluids.

Keratocan Antibody

R31830 100 ug
EUR 356.15
Description: Keratocan (KTN), also known as keratan sulfate proteoglycan keratocan, is a protein that in humans is encoded by the KERA gene. It is mapped to 12q22. The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2). Keratan sulfate proteoglycans (KSPGs) are members of the small leucine-rich proteoglycan (SLRP) family. KSPGs, particularly keratocan, lumican and mimecan, are important to the transparency of the cornea.

Rabbit Keratocan (KERA) ELISA Kit

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Bovine Keratocan, KERA ELISA KIT

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ELISA kit for Human KERA (Keratocan)

ELK3438 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Keratocan from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

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20-abx167289
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  • EUR 343.20
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  • 100 ug
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  • 50 ug

Human Keratocan (KERA) Protein

20-abx654110
  • EUR 693.60
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  • EUR 2064.00
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  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Monkey Keratocan (KERA) ELISA Kit

abx354947-96tests 96 tests
EUR 990

ELISA kit for Human KERA (Keratocan)

E-EL-H1794 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human KERA (Keratocan) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Keratocan (KERA)

KTE61923-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Keratocan (KERA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Keratocan (KERA)

KTE61923-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Keratocan (KERA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Keratocan (KERA)

KTE61923-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Keratocan (KERA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Ktcn Antibody / Kera / Keratocan

RQ7006 100 ug
EUR 356.15
Description: Keratocan (KTN) also known as keratan sulfate proteoglycan keratocan, is a protein that in humans is encoded by the KERA gene. The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).

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Chicken Keratocan (KERA) ELISA Kit

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4-RPC553Bo01
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  • 100 ug
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  • 50ug
  • 5 mg
Description: Recombinant Bovine Keratocan expressed in: E.coli

Recombinant Keratocan (KERa)

4-RPC553Mu01
  • EUR 582.34
  • EUR 279.60
  • EUR 1853.76
  • EUR 697.92
  • EUR 1275.84
  • EUR 465.60
  • EUR 4454.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Keratocan expressed in: E.coli

Keratocan (KERA) Polyclonal Antibody (Bovine)

4-PAC553Bo01
  • EUR 331.20
  • EUR 3566.40
  • EUR 876.00
  • EUR 422.40
  • EUR 271.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA)

Keratocan (KERA) Polyclonal Antibody (Bovine), PE

4-PAC553Bo01-PE
  • EUR 397.20
  • EUR 3772.80
  • EUR 1051.20
  • EUR 506.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA). This antibody is labeled with PE.

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat)

4-PAC553Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA)

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat), PE

4-PAC553Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA). This antibody is labeled with PE.

Keratocan (KERA) Polyclonal Antibody (Bovine), APC

4-PAC553Bo01-APC
  • EUR 466.80
  • EUR 4686.00
  • EUR 1284.00
  • EUR 603.60
  • EUR 285.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA). This antibody is labeled with APC.

Keratocan (KERA) Polyclonal Antibody (Bovine), Cy3

4-PAC553Bo01-Cy3
  • EUR 572.40
  • EUR 6198.00
  • EUR 1662.00
  • EUR 754.80
  • EUR 331.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA). This antibody is labeled with Cy3.

Keratocan (KERA) Polyclonal Antibody (Bovine), HRP

4-PAC553Bo01-HRP
  • EUR 424.80
  • EUR 4081.20
  • EUR 1132.80
  • EUR 542.40
  • EUR 267.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA). This antibody is labeled with HRP.

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat), APC

4-PAC553Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA). This antibody is labeled with APC.

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat), Cy3

4-PAC553Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA). This antibody is labeled with Cy3.

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat), HRP

4-PAC553Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA). This antibody is labeled with HRP.

Keratocan (KERA) Polyclonal Antibody (Bovine), FITC

4-PAC553Bo01-FITC
  • EUR 397.20
  • EUR 3772.80
  • EUR 1051.20
  • EUR 506.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA). This antibody is labeled with FITC.

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat), FITC

4-PAC553Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA). This antibody is labeled with FITC.

Keratocan (KERA) Polyclonal Antibody (Bovine), APC-Cy7

4-PAC553Bo01-APC-Cy7
  • EUR 790.80
  • EUR 9228.00
  • EUR 2424.00
  • EUR 1063.20
  • EUR 427.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA). This antibody is labeled with APC-Cy7.

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat), APC-Cy7

4-PAC553Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA). This antibody is labeled with APC-Cy7.

Keratocan (KERA) Polyclonal Antibody (Bovine), Biotinylated

4-PAC553Bo01-Biotin
  • EUR 412.80
  • EUR 3506.40
  • EUR 1011.60
  • EUR 512.40
  • EUR 279.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Keratocan (KERA). This antibody is labeled with Biotin.

Keratocan (KERA) Polyclonal Antibody (Mouse, Rat), Biotinylated

4-PAC553Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Keratocan (KERA). This antibody is labeled with Biotin.

DURAN Hydra Square GL45 Purging Bottle Clear 1000mL with 4x GL45 Side Necks

BOT2570 EACH
EUR 351.6

Cellufine PB

683-986-324 10 ml
EUR 542.4

Cellufine PB

683-986-326 50 ml Ask for price

Cellufine PB

683-986-328 500 ml Ask for price

Cellufine PB

683-986-330 5 lt Ask for price

Cellufine PB

683-986-335 10 lt Ask for price

PB antibody

70R-2192 50 ug
EUR 560.4
Description: Rabbit polyclonal PB antibody raised against the middle region of Pb

Lead (Pb)

AT010 1mg
EUR 1641.6

Pseudoproto-Pb

TBW00844 unit Ask for price

PB Cadherin Antibody

47938-100ul 100ul
EUR 302.4

MC Cellufine PB

202-15 1 x 5 ml
EUR 542.4

MC Cellufine PB

202-51 5 x 1 ml
EUR 542.4

PB Blocking Peptide

33R-2067 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of pb antibody, catalog no. 70R-2192

PB 28 dihydrochloride

B7107-10 10 mg
EUR 340.8

PB 28 dihydrochloride

B7107-50 50 mg
EUR 1245.6

PB Cadherin Conjugated Antibody

C47938 100ul
EUR 476.4

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